| Catalog Number | TM-901Y-55 |
| Product Id | TM-901Y-55 |
| Product Name | Anti-human Mediator 10 (MED10 or HNUT2) IgG fraction(monoclonal) |
| Temperature | -20°C |
| Unit | 150µg |
| Price | 425 |
| Availability | Available |
| Category | Proteins and Antibodies for Transcription Research |
| Subcategory | Proteins and Antibodies |
| Description | IgG fraction from mouse monoclonal antibody (clone 7D11/H4) obtained by immunization of mice with human MED10 (25 kDa). The IgG fraction was purified using Protein G-Sepharose. |
| Isotype | IgG2a |
| Purity | N/A |
| Stability | Stable for at least one year. Avoid repeated freezing and thawing |
| Biological Activity | This antibody can be used in ELISA assays (1: 8,000 dilution) and Western blot. It recognizes human MED10 (hNut2), a subunit protein of Mediator (MED) complex. The Mediator is a multiprotein coactivator required for activation of RNA polymerase II transcription by DNA binding transcription factors. The MED10 has been assigned to the middle domain of Mediator and it interacts physically with the carboxy-terminal domain (CTD) of largest RNA polymerase II subunit according to electron microscopic data. |
| Formulation | Solution at 1.0 mg/mL in PBS |
| References | Guglielmi, B. al (2004) Nucleic Acids Res. 32, 5379 – 5391, Bourbon, H-M. et al (2004) Mol Cell 14, 553 – 557, Lewis, B. & Reinberg, D. (2003) J. Cell Sci. 116, 3667 – 3675, Boube, M. et al (2002) Cell 110, 143 – 151, Woychik, N. & Hampsey, M. (2002) Cell 108, 453 – 463. |
