| Catalog Number | TM-1411W-55 |
| Product Id | TM-1411W-55 |
| Product Name | Anti-human Di/Tri-Methyl K27 Histone h3 (DI/TRI-Methylated H3 K27) IgG fraction |
| Temperature | -20°C |
| Unit | 150µg |
| Price | 425 |
| Availability | Available |
| Category | Proteins and Antibodies for Transcription Research |
| Subcategory | Proteins and Antibodies |
| Description | IgG fraction from mouse monoclonal antibody (clone 7B11/G5) obtained by immunization of mice with a KLH-conjugated synthetic peptide TKAAR[dimethyl-K]SAP-C corresponding to aa22 – aa30 of human histone H3. The IgG fraction was purified using Protein G-Sepharose. |
| Isotype | IgG-3 |
| Purity | N/A |
| Storage | Store at -20°C. |
| Stability | Stable for at least one year. Avoid repeated freezing and thawing |
| Biological Activity | This antibody can be used in ELISA assays (1: 1,000 dilution) and immunofluorescence assays. It recognizes di- and trimethylated H3 K27. The methylation of specific histone lysines controls aspects of short- and long-term transcriptional regulation, in addition to the propagation of bulk chromosome structure and stability. Methylated histone H3 K27 is also involved in transcriptional silencing of human polycomb target genes and earliest chromatin changes in the X-chromosome inactivation. Methylated histone H3 K27 is considered a mark of repressive chromatin states |
| Formulation | Solution at 1.0 mg/mL in PBS |
| References | Hampsey, M & Reinberg, D. (2003) Cell 113, 429 – 432; Rougeulle, C. et. al. (2004) Mol. Cell. Biol. 24, 5475 – 5484; Kirmizis, A. et. al. (2004) Genes & Dev. 18, 1592 – 1605; Sims, R. J. et. al. (2003) Trends Genet. 19, 629 – 639. |
