| Catalog Number | PCA-2015a-9 |
| Product Id | PCA-2015a-9 |
| Product Name | Rabbit anti-Human Cullin4a (CUL 4a) IgG fraction polyclonal antibody |
| Temperature | -20°C |
| Unit | 1mg |
| Price | 425 |
| Availability | Available |
| Category | Growth Factors, Cytokines, Receptors and their Antibodies |
| Subcategory | Polyclonal Antibodies (Cancer/Apoptosis) |
| Description | IgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide (MADEAPRKGSFSALVGRTNG) corresponding to amino acids 1-20 of human Cullin 4a. The IgG fraction was purified using Protein G-Sepharose. |
| Isotype | N/A |
| Mol Weight | N/A |
| Purity | N/A |
| Storage | Store at -20ºC. |
| Stability | Stable at least one year at -20ºC. Avoid repeated freezing and thawing. |
| Biological Activity | This polyclonal antibody can be used in ELISA (1: 500 dilution), immunocitochemistry (1:100 dilution) and it recognizes Cullin 4A (75 kDa) in Western blot of HeLa lysate (1: 1,000 dilution). Cullins (1, 2, 3, 4a, 5 and 7) are a family of proteins that assemble a large number of ubiquitin ligases to catalyse polyubiquitiation, as well as binding to various specific factors to recruit substrates. Cullin 4 (CUL 4) serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits of SCF. It plays a role in the polyubiquitination of CDT1 in response to radiation-induced DNA damage and may be important for subsequent DNA repair. |
| Formulation | Solution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial. |
| References | Chen, L-C. et al. (1998) Cancer Res. 58, 3677-3683. Dai, Q. & Wang, H. (2006) Cell Div. 1, 14. Higa, L.A et al. (2006) Cell Cycle 5, 1675-1680. Angers, S. et al. (2006) Nature 443, 590-593. |
