| Catalog Number | PCA-2020a-9 |
| Product Id | PCA-2020a-9 |
| Product Name | Rabbit anti-Human Cullin5 (CUL 5) IgG fraction polyclonal antibody |
| Temperature | -20°C |
| Unit | 1mg |
| Price | 425 |
| Availability | Available |
| Category | Growth Factors, Cytokines, Receptors and their Antibodies |
| Subcategory | Polyclonal Antibodies (Cancer/Apoptosis) |
| Description | IgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide (SNLLKNKGSLQFEDK) corresponding to amino acids 4-19 of human Cullin 5. The IgG fraction was purified using Protein G-Sepharose. |
| Isotype | N/A |
| Mol Weight | N/A |
| Purity | N/A |
| Storage | Store at -20ºC. |
| Stability | Stable at least one year at -20ºC. Avoid repeated freezing and thawing. |
| Biological Activity | This polyclonal antibody can be used in ELISA (1: 500 dilution), immunocitochemistry (1:100 dilution) and it recognizes Cullin 5 (91 kDa) in Western blot of HeLa lysate (1: 1,000 dilution). Cullins (1, 2, 3, 4a, 5 and 7) are a family of proteins that assemble a large number of ubiquitin ligases to catalyse polyubiquitiation, as well as binding to various specific factors to recruit substrates. Cullin 5 (CUL 5) is a component of E3 ubiquitin ligase complexes and it is involved in proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. It may also form a cell surface vasopresin receptor and it may play a role in brast tumorigenesis. |
| Formulation | Solution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial. |
| References | Byrd, P.J. et al. (1997) Genome Res. 7, 71-75. Fay, M.J. et al. (2003) Mol. Cancer 2, 40, |
