| Catalog Number | PCA-2070a-9 |
| Product Id | PCA-2070a-9 |
| Product Name | Rabbit anti-Human B-cell Leukaemia/lymphoma 2 (Bcl-2) IgG fraction polyclonal antibody |
| Temperature | -20°C |
| Unit | 1mg |
| Price | 425 |
| Availability | Available |
| Category | Growth Factors, Cytokines, Receptors and their Antibodies |
| Subcategory | Polyclonal Antibodies (Cancer/Apoptosis) |
| Description | IgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide (GAAPAPGIFSSQPG) corresponding to amino acids 41 - 54 of human Bcl-2. The IgG fraction was purified using Protein G-Sepharose. |
| Isotype | N/A |
| Mol Weight | N/A |
| Purity | N/A |
| Storage | Store at -20ºC. |
| Stability | Stable at least one year at -20ºC. Avoid repeated freezing and thawing. |
| Biological Activity | This polyclonal antibody reacts in ELISA using the immunizing peptide and it recognizes a 25 kDa protein in Western Blot of extract from Jurkat cells. Bcl-2 is a protein localized on the mitochondrial outer membrane, endoplasmic reticulum, and nuclear envelope. It supresses apoptosis modulated by homodimerization or association with Bax and Bad, two cell death promoters. It may contribute to tumorigenesis by prolonging cell survival and by mutations in the Bcl-2 gene. |
| Formulation | Solution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial. |
| References | Reed, J.C. (1997) Nature 387, 773 - 776. Chan, S.L. & Yu, V.C. (2004) Clin. Exp. Pharmacol. Physiol. 31, 119 - 128. Sorenson, C.M. (2004) Biochim. Biophys. Acta 1644, 169 - 177. Kirkin, V. et al. (2004) Biochim. Biophys. Acta 1644, 229 - 249. |
