| Catalog Number | PCA-2075a-9 |
| Product Id | PCA-2075a-9 |
| Product Name | Rabbit anti-Human Bax IgG fraction polyclonal antibody |
| Temperature | -20°C |
| Unit | 1mg |
| Price | 425 |
| Availability | Available |
| Category | Growth Factors, Cytokines, Receptors and their Antibodies |
| Subcategory | Polyclonal Antibodies (Cancer/Apoptosis) |
| Description | IgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide (MDGSGEQPRGGGPTSSEQIMK) corresponding to amino acids 1 - 21 of human Bax. The IgG fraction was purified using Protein G-Sepharose. |
| Isotype | N/A |
| Mol Weight | N/A |
| Purity | N/A |
| Storage | Store at -20ºC. |
| Stability | Stable at least one year at -20ºC. Avoid repeated freezing and thawing. |
| Biological Activity | This polyclonal antibody reacts in ELISA using the immunizing peptide and it recognizes two protein bands corresponding to 20 (monomer) and 40 kDa (dimer) of Bcl-2 in Western Blot of HL-60 cell lysate. Bax belongs to Bcl-2 protein family and accelerates the apoptosis by binding to the repressor Bcl-2. It can form oligomers and targets mitochondrial membrane inducing an increase in the membrane permeability which leads to the release of cytochrome c from mitochondria and the activation of the caspase pathway. Bax is considered a tumor suppressor. |
| Formulation | Solution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial. |
| References | Oltvai, Z.N. et al. (1993) Cell 74, 609-619. Reed, J.C. (1994) J. Cell Biol. 124, 1-6. Wei, M.C. (2001) Science 292, 727-720. |
