| Catalog Number | PCA-2110a-9 |
| Product Id | PCA-2110a-9 |
| Product Name | Rabbit anti-Human NFkappaB p50 IgG fraction polyclonal antibody |
| Temperature | -20°C |
| Unit | 1mg |
| Price | 425 |
| Availability | Available |
| Category | Growth Factors, Cytokines, Receptors and their Antibodies |
| Subcategory | Polyclonal Antibodies (Cancer/Apoptosis) |
| Description | IgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide (MAEDDPYLGRPE) corresponding to amino acids 1-12 of human NFkappaB p50. The IgG fraction was purified using Protein G-Sepharose. |
| Isotype | N/A |
| Mol Weight | N/A |
| Purity | N/A |
| Storage | Store at -20ºC. |
| Stability | Stable at least one year at -20ºC. Avoid repeated freezing and thawing. |
| Biological Activity | This polyclonal antibody reacts in ELISA and Western blot using the immunizing peptide (1: 500 dilution). Nuclear factor kappa light chain enhancer in B cells (NFkappaB) is a transcription regulator that consists of two subunits, 50 kDa DNA binding subunit (p50) and 65 kDa transactivation subunit (p65/Rel1A). p50 subunit has an isoform p52 and both proteins are derived from p105 precursor.The p50/p65 heterodimer remains in the cytosol in an inactive form bounded to its inhibitor IkappaB. Upon stimulation, phosphorylation of IkappaB releases NFkappaB complex which is traslocated into de nucleus and regulates the expression of genes that participate in immune, apoptotic, and oncogenic processes. |
| Formulation | Solution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial. |
| References | Lin, L. & Ghosh, S. (1996) Mol. Cell. Biol. 16, 2248-2254. Bassères, D.S. & Baldwin, A.S., (2006) Oncogene 25, 6017-6830. |
