| Catalog Number | PCA-2130a-9 |
| Product Id | PCA-2130a-9 |
| Product Name | Rabbit anti-Human Survivin IgG fraction polyclonal antibody |
| Temperature | -20°C |
| Unit | 1mg |
| Price | 425 |
| Availability | Available |
| Category | Growth Factors, Cytokines, Receptors and their Antibodies |
| Subcategory | Polyclonal Antibodies (Cancer/Apoptosis) |
| Description | IgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide (EETAKKVRRAIEQLAAMD) corresponding to amino acids 125-142 of human Survivin. The IgG fraction was purified using Protein G-Sepharose. |
| Isotype | N/A |
| Mol Weight | N/A |
| Purity | N/A |
| Storage | Store at -20ºC. |
| Stability | Stable at least one year at -20ºC. Avoid repeated freezing and thawing. |
| Biological Activity | This polyclonal antibody reacts in ELISA (1: 50 dilution) and Western blot (1: 1,000) using the immunizing peptide. Survivin is a 16 kDa apoptosis inhibitor protein (IAP). It binds and inhibits the caspase-3 proteolytic activity and controls the checkpoint in the G2/M phase of the cell cycle to promote cell division by the control of microtubule stability and assembly of a normal mitotic spindle. Survivin is highly expressed during fetal development, transformed cell lines, human cancers and non-Hodgkin's lymphomas. |
| Formulation | Solution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial. |
| References | Ambrosini, G. et al. (1997) Nat. Med. 3, 917-921. Li, F. et al. (1998) Nature 396, 580-584. Verhagen, A.M. et al. (2001) Genome Biol. 2, 3009.1-3009.10. Giodini, A. et al. (2002) Cancer Res. 62, 2462-2467. Ryan, B.M. et al. (2006) Ann. Oncol. 17, 597-604. |
