| Catalog Number | PCA-3010a-9 |
| Product Id | PCA-3010a-9 |
| Product Name | Rabbit anti-Human Cyclin-dependent Kinase 4 (Cdk4) IgG fraction polyclonal antibody |
| Temperature | -20°C |
| Unit | 1mg |
| Price | 425 |
| Availability | Available |
| Category | Growth Factors, Cytokines, Receptors and their Antibodies |
| Subcategory | Polyclonal Antibodies (Cancer/Apoptosis) |
| Description | IgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide (QHSYLHKDEGNPE) corresponding to amino acids 291-303 of human Cdk4. The IgG fraction was purified using Protein G-Sepharose. |
| Isotype | N/A |
| Mol Weight | N/A |
| Purity | N/A |
| Storage | Store at -20ºC. |
| Stability | Stable at least one year at -20ºC. Avoid repeated freezing and thawing. |
| Biological Activity | This polyclonal antibody reacts in ELISA (1: 250 dilution) and Western blot (1: 1,000 dilution) using the immunizing peptide. Cyclin-dependent kinase 4 (Cdk4) is a 34 kDa catalytic subunit of the protein kinase complex and belongs to the Ser/Thr protein kinase family. Cdk4 forms a complex with D-type cyclins and may regulate cell growth during G1 phase of the cell cycle. Cdk4 also associates with Cdk inhibitor p16 that down-regulates Cdk4 activity. Mutations in Cdk4 are associated with tumorigenesis of a variety of cancers. |
| Formulation | Solution at 1.0 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial. |
| References | Khatib, Z.A. et al. (1993) Cancer Res. 53, 5535-5541. Pines, J. (1995) Biochem. J. 308, 697-711. Bockstaele, L. et al. (2006) Cell Div. 1: 25. |
