| Catalog Number | PCA-3040a-9 |
| Product Id | PCA-3040a-9 |
| Product Name | Rabbit anti-Human S-phase Kinase-associated protein 1A (Skp1) IgG fraction polyclonal antibody |
| Temperature | -20°C |
| Unit | 1mg |
| Price | 425 |
| Availability | Available |
| Category | Growth Factors, Cytokines, Receptors and their Antibodies |
| Subcategory | Polyclonal Antibodies (Cancer/Apoptosis) |
| Description | IgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide (AQVRKENQWCEEK) corresponding to amino acids 151-163 of human Skp1 . The IgG fraction was purified using Protein G-Sepharose. |
| Isotype | N/A |
| Mol Weight | N/A |
| Purity | N/A |
| Storage | Store at -20ºC. |
| Stability | Stable at least one year at -20ºC. Avoid repeated freezing and thawing. |
| Biological Activity | This polyclonal antibody reacts in ELISA (1: 250 dilution) and Western blot (1:1,000 dilution) using the immunizing peptide. S-phase kinase-associated protein 1A (Skp1) is a component of the SCF ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. Skp1 interacts directly with cullin 1 and F-box proteins in the SCF complex. It is also involved in the control of beta-catenin levels and the activity of beta-catenin dependent TCF transcription factors. |
| Formulation | Solution at 1.0 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial. |
| References | Schulman, B.A. et al. (2000) Nature 408, 381-386. Zheng, N. et al. (2002) Nature 416, 703-709. Cardoso, T. & Pagano, M. (2006) Nat. Rev. Mol. Cell Biol. 5, 739-751. |
