| Catalog Number | PN-7090a-9 |
| Product Id | PN-7090a-9 |
| Product Name | Rabbit anti-Human Early Endosome Antigen 1 (EEA1) IgG fraction polyclonal antibody |
| Temperature | -20°C |
| Unit | 1mg |
| Price | 425 |
| Availability | Available |
| Category | Growth Factors, Cytokines, Receptors and their Antibodies |
| Subcategory | Polyclonal Antibodies (Neurobiology) |
| Description | IgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide (TPSSKKPVRVCDACFNDLQG) corresponding to amino acids 1392-1411 of human EEA1. The IgG fraction was purified using Protein G-Sepharose. |
| Isotype | N/A |
| Mol Weight | N/A |
| Purity | N/A |
| Storage | Store at -20ºC. |
| Stability | Stable at least one year at -20ºC. Avoid repeated freezing and thawing. |
| Biological Activity | This polyclonal antibody can be used in ELISA (1: 50 dilution), immunocytochemistry (1: 100 dilution) and it recognizes EEA1 (162 kDa) in Western blot of HeLa lysate (1: 500 dilution). Early endosome antigen 1 (EEA1) is a peripheral membrane protein that contains a calmodulin-binding IQ motif and cysteine rich finger motif necessary for its specific localization to the early endosomes. These early endosomes are cytoplasmatic compartments which fuse the endocytic vesicles for redistribution of extracellular compounds to alternate destinations. EEA1 mediates the regulatory effects of 3'-phosphoinositides on membrane trafficking through its FYVE-type zinc finger domain. |
| Formulation | Solution at 0.5 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial. |
| References | Mu, F.T. et al. (1995) J. Biol. Chem. 270, 13503-13511. Stenmark, H. et al. (1996) J. Biol. Chem. 271, 24048-24054. Gaullier, J.M. et al. (2000) J. Biol. Chem. 275, 24595-24600. |
