| Catalog Number | FM-122AX-5 |
| Product Name | Anti-Infectious salmon anemia virus (ISAV) Hemagglutinin-Esterease protein. IgG fraction monoclonal antibody (clone 8D2/E9) |
| Temperature | Select |
| Unit | 100µg |
| Price | 425 |
| Availability | Available |
| Category | Infectious Agents |
| Subcategory | Fish Pathogens (Aquaculture) |
| Description | Mouse monoclonal antibody IgG fraction (clone 8D2/E9) obtained by immunizing mice with a purified N-terminal hemagglutinin recombinant fragment of ISAV. The IgG fraction was purified using Protein G-Sepharose. |
| Isotype | Select |
| Mol Weight | N/A |
| Purity | N/A |
| Storage | Store at -20ºC. |
| Stability | Stable at least one year at -20ºC. Avoid repeated freezing and thawing. |
| Biological Activity | This antibody can be used in ELISA (1:500 dilution), Western blot (1:1,000 dilution), and indirect immunofluorescence (1:1,000). It recognizes two bands, one at 42 kDa corresponding to ISAV hemagglutinin-esterase and the second at 90 kDa probably corresponding to a dimmer of this protein. ISAV is an enveloped virus that belongs to the family Orthomyxoviridae and genus Isavirus. Its genoma comprises eight negative-sense, single-stranded RNA segments and encodes four major structural proteins including the matrix, the nucleoprotein, and two membrane glycoproteins (hemagglutinin-esterase and gp50). ISAV infects mainly endothelial cells and leukocytes and causes a multisystemic disease characterized by high mortality with exophthalmia, pale gills, ascites, hemorrhagic liver necrosis, and renal interstitial hemorrhage. |
| Formulation | Solution at 1.0 mg/mL in PBS. |
| References | Kross?y, B. et al. (2001) J. Gen. Virol. 82, 1757-1765. Rimstad, E. & Mjaaland, S. (2002) APMIS 110, 273-282. Falk,K. et al. (2004) J. Virol. 78, 3063-3071. Aspehaug, V. et al. (2004) Virus Res. 106, 51-60. Goic, B. et al. (2008) Virology 379, 55-63. |
