| Catalog Number | HNM-6031AZ1-5 |
| Product Name | Anti-Nucleoprotein of Dobrava Virus (DOBV). IgG fraction monoclonal antibody. Clone 1E9/G2 |
| Temperature | -20°C |
| Unit | 100µg |
| Price | 499 |
| Availability | Available |
| Category | Infectious Agents |
| Subcategory | Hantavirus |
| Description | Mouse monoclonal antibody IgG fraction (clone 1E9/G2) obtained by immunizing mice with purified recombinant DOBV Nucleoprotein. The IgG fraction was purified using Protein G-Sepharose. |
| Isotype | IgG-1 |
| Mol Weight | N/A |
| Purity | N/A |
| Storage | Store at -20ºC. |
| Stability | Stable at least one year at -20ºC. Avoid repeated freezing and thawing. |
| Biological Activity | This antibody can be used in ELISA (1:30,000 dilution) and Western blot (1:10,000 dilution) analysis. It recognizes specifically the nucleoprotein of DOBV. Hantaviruses (family Bunyaviridae) are enveloped viruses that contain a tripartite, negative-stranded RNA genome encoding an RNA-dependent RNA polymerase (L), two integral membrane glycoproteins (G1 and G2), and a nucleocapsid (N) protein. The N protein is the most abundant viral component and the major antigen in early serological response in humans and mice. It has essential functions in viral RNA replication, encapsidation, and also in virus assembly. DOBV causes hemorrhagic fever with renal syndrome in eastern and central Europe and is transmitted by two different rodent species, Apodemus flavicollis and Apodemus agrarius. |
| Formulation | Solution at 1.0 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial. |
| References | Klempa, B. et al. (2004) J. Clin. Microbiol. 42, 1322-1325.
Vapalahti, O. et al (2003) Lancet Infect. Dis. 3, 653-661.
Sibold, C. et al (2001) J. Med. Virol. 63, 158-167.
Avsic-Zupanc, T. et al. (1995) J. Gen. Virol. 76, 2801-2808. |
