| Catalog Number | HNM-6032BW1-5 |
| Product Name | Anti-Glycoprotein 1 of Dobrava Virus (DOBV). IgG fraction monoclonal antibody. Clone 6D4/F12 |
| Temperature | -20°C |
| Unit | 100µg |
| Price | 499 |
| Availability | Available |
| Category | Infectious Agents |
| Subcategory | Hantavirus |
| Description | Mouse monoclonal antibody IgG fraction (clone 6D4/F12) obtained by immunizing mice with purified recombinant DOBV Glycoprotein 1 (G1). The IgG fraction was purified using Protein G-Sepharose. |
| Isotype | IgG-3 |
| Mol Weight | N/A |
| Purity | N/A |
| Storage | Store at -20ºC. |
| Stability | Stable at least one year at -20ºC. Avoid repeated freezing and thawing. |
| Biological Activity | This antibody can be used in ELISA (1:8,000 dilution) and Western blot (1:8,000 dilution) analysis. It recognizes G1 glycoprotein of DOBV and it has cross reactivity with G1 glycoprotein of Seoul virus (SEOV). Hantaviruses (family Bunyaviridae) are enveloped viruses that contain a tripartite, negative-stranded RNA genome encoding an RNA-dependent RNA polymerase (L), two integral membrane glycoproteins (G1 and G2), and a nucleocapsid (N) protein. The G1 and G2 glycoproteins form heterodimers and they attach the virion to a cell receptor and to promote fusion membrane after endocytosis of the virion. G1 can dysregulate normal immune and endothelial cell responses and contribute to virus pathogenesis. DOBV causes hemorrhagic fever with renal syndrome in eastern and central Europe and is carried by two different rodent species, Apodemus flavicollis and Apodemus agrarius. |
| Formulation | Solution at 0.5 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial. |
| References | Klempa, B. et al. (2004) J. Clin. Microbiol. 42, 1322-1325.
Vapalahti, O. et al (2003) Lancet Infect. Dis. 3, 653-661.
Sibold, C. et al (2001) J. Med. Virol. 63, 158-167.
Avsic-Zupanc, T. et al. (1995) J. Gen. Virol. 76, 2801-2808. |
