| Catalog Number | AP-30103-9 |
| Product Name | Rabbit anti-Alfalfa Mosaic Virus (AMV) IgG fraction POLYCLONAL |
| Temperature | -20°C |
| Unit | 1mg |
| Price | 425 |
| Availability | Available |
| Category | Infectious Agents |
| Subcategory | Plant Pathogens (Agriculture) |
| Description | Rabbit polyclonal antibody IgG fraction obtained by immunizing rabbits with a mix of two synthetic peptides corresponding to aa14-27 (KPTKRSQNYAALRK), and aa193-205 (ERKAFPRAGPLRS) of coat protein from AMV. The IgG fraction was purified using Protein G-Sepharose. |
| Isotype | N/A |
| Mol Weight | N/A |
| Purity | N/A |
| Storage | Store at -20ºC. |
| Stability | Stable at least one year at -20ºC. Avoid repeated freezing and thawing. |
| Biological Activity | This polyclonal antibody reacts with extracts of AMV-infected plants in PTA (Plate-Trapped Antigen)-ELISA and Western Blot assays (1: 50 dilution). AMV is a positive-strand RNA virus with tripartite genome belonging to the family Bromoviridae. It forms bacilliform or spherical particles and is transmitted in a non-persistent manner by numerous aphids species. The AMV genome is encapsidated by a single coat protein (24 kDa) which plays a key role in early and late AMV infection, functioning in genome activation, RNA replication, and in cell-to-cell systemic movement of virus. |
| Formulation | Solution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial. |
| References | Herranz, M.C. et. al. (2012) Mol. Plant Microbe Interact. 25, 1093-1103.
Bol, J.F. (2005) Annu. Rev. Phytopathol. 43, 39 - 62.
Bol, J.F (2003) Mol. Plant Pathol. 4, 1 - 8. |
