| Catalog Number | AP-1403-9 |
| Product Name | Rabbit Anti-Grapevine Leafroll-Associated Virus 1 (GLRaV-1) Coat Protein (petides) IgG fraction POLYCLONAL |
| Temperature | -20°C |
| Unit | 1mg |
| Price | 425 |
| Availability | Available |
| Category | Infectious Agents |
| Subcategory | Plant Pathogens (Agriculture) |
| Description | IgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a mix of six synthetic peptides corresponding to aa6-19 (SQNDDDYNVVRSGNK), aa88-100 (SRPSTGQRFGDSEK), aa103-115 (RREMGDKLKRTFN), aa20-220 (YSNGERKLTYEKVK), aa259-261 (NEKVAMQHGVPKR), and aa310-322 (SSVL RNTGELKV) of coat protein from GLRaV-1. The IgG fraction was purified using Protein G-Sepharose. |
| Isotype | N/A |
| Mol Weight | N/A |
| Purity | N/A |
| Storage | Store at -20ºC. |
| Stability | Stable at least one year at -20ºC. |
| Biological Activity | This polyclonal antibody reacts with extracts of GLRaV-1-infected plants in ELISA, PTA (Plate-Trapped Antigen)-ELISA, and Western blot (1: 200 dilution). GLRaV-1 belongs to the genus Ampelovirus of the family Closteroviridae. Its genome consists of a single-stranded positive-sense RNA and is encapsidated in filamentous particles containing a 35 kDa coat protein. GLRaV-1 is transmitted by vegetative propagation, grafting, scale insect, and mealybugs. It has been reported that GLRaV-1 has a great potential for genetic variation. |
| Formulation | Solution at 1 mg/mL in PBS. Avoid repeated freezing and thawing. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial. |
| References | Alabi, O.J. et. al. (2011) Phytopathology 101, 1446-1456.
Lanza, M. et. al. (2010) Trop. Plant Pathol. 35, 377-380.
Little, A. et. al. (2001) Virus Res. 80, 109-116. |
