| Catalog Number | AP-2103-9 |
| Product Name | Rabbit Anti-Prunus Necrotic Ringspot Virus (PNRSV) IgG fraction POLYCLONAL |
| Temperature | -20°C |
| Unit | 1mg |
| Price | 425 |
| Availability | Available |
| Category | Infectious Agents |
| Subcategory | Plant Pathogens (Agriculture) |
| Description | IgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a mixture of three synthetic peptides corresponding to aa18-34 (KRCHPNDALV PLRAQQR), aa 08-118 (QLMGQNLTLLT), and aa165-177 (EPPSDLDFDTFAR) of the PNRSV coat protein. The IgG fraction was purified using Protein G-Sepharose. |
| Isotype | N/A |
| Mol Weight | N/A |
| Purity | N/A |
| Storage | Store at -20ºC. |
| Stability | Stable at least one year at -20ºC. Avoid repeated freezing and thawing. |
| Biological Activity | This polyclonal antibody reacts with extracts of PNRSV-infected plants in PTA (Plate-Trapped Antigen)-ELISA (1: 50 dilution). PNRSV belongs to the genus Ilarvirus of the family Bromoviridae, its genome consists of the three segments of positive single-stranded RNA molecules. The PNRSV coat protein (25 kDa) is required for RNA accumulation, encapsidation, cell-to-cell movement, and systemic spread of the virus. PNRSV is a isometric virus and is transmitted by grafting and seeds. |
| Formulation | Solution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial. |
| References | Fiore, N. et. al. (2008) Arch. Virol. 153, 909-919.
Codoñer, F.M. et. al.(2006) J. Gen. Virol. 80, 5833-5840.
Aparicio, F. et. al. (2003) Virology 313, 213-223. |
