| Catalog Number | AP-10103-9 |
| Product Name | Rabbit Anti-Plum Pox Virus (PPV) IgG fraction POLYCLONAL |
| Temperature | -20°C |
| Unit | 1mg |
| Price | 425 |
| Availability | Available |
| Category | Infectious Agents |
| Subcategory | Plant Pathogens (Agriculture) |
| Description | IgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a mixture of three synthetic peptides corresponding to aa86-96 (STGTFTVPRLK), aa146-158 (KRDYDVTDDEMSI), and aa242-255 (SLARYAFDFYEMTS) of the PPV coat protein. The IgG fraction was purified using Protein G-Sepharose. |
| Isotype | N/A |
| Mol Weight | N/A |
| Purity | N/A |
| Storage | Store at -20ºC. |
| Stability | Stable at least one year at -20ºC. Avoid repeated freezing and thawing. |
| Biological Activity | This polyclonal antibody reacts with extracts of PPV-infected plants in DAS-ELISA as a capture antibody (1: 2,000 dilution) and Western blot assay (1: 500 dilution). PPV belongs to the genus Potyvirus of the family Potyviridae and is the causal agent of sharka disease of stone fruits crops. PPV genome consists of a single-stranded, positive-sense RNA encapsidated by a single glycosylated coat protein (34 kDa). This protein is also involved in systemic movement of the virus. PPV has flexuous filamentous morphology and is transmitted by an insect and grafting. |
| Formulation | Solution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial. |
| References | Fernández-Fernández, M.R. et. al. (2002) J. Biol. Chem. 277, 135-140.
Riechmann, J.L. et. al. (1992) J. Gen. Virol. 73, 1-6.
Laín, S. et. al. (1989) Virus Res. 13, 157-172. |
