| Catalog Number | AP-5103-9 |
| Product Name | Rabbit Anti-Raspberry Bushy Dwarf Virus (RBDV) IgG fraction POLYCLONAL |
| Temperature | -20°C |
| Unit | 1mg |
| Price | 425 |
| Availability | Available |
| Category | Infectious Agents |
| Subcategory | Plant Pathogens (Agriculture) |
| Description | IgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a mixture of three synthetic peptides corresponding to aa11-24 (KAQYELYNRKL NRA), aa149-161 (TFNDLAAQKAGDK), and aa202-212 (EAINLVSGLNK) of the RBDV coat protein. The IgG fraction was purified using Protein G-Sepharose. |
| Isotype | N/A |
| Mol Weight | N/A |
| Purity | N/A |
| Storage | Store at -20ºC. |
| Stability | Stable at least one year at -20ºC. Avoid repeated freezing and thawing. |
| Biological Activity | This polyclonal antibody reacts with extracts of RBDV infected plants in PTA (Plate-Trapped Antigen)-ELISA (1: 200 dilution) and it recognizes a band (30 kDa) correponding to RBDV coat protein by Western Blot. RBDV belongs to the genus Idaeovirus,its genome consists of two segments of positive single-stranded RNA molecules. The genome is packaged in isometric particles with a coat protein of 30 kDa. RBDV infects wild and cultivated Rubus species and is transmitted by pollen and seeds. |
| Formulation | Solution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial. |
| References | MacFarlane, S. & McGavin, W. (2009) J. Gen. Virol. 90, 747-753. Natsuaki, T. et. al. (1991) J. Gen. Virol. 72, 2183-2189. Jones, A. T. et. al. (2000) Eur. J. Plant Pathol. 106, 623-632. |