| Catalog Number | GF-010-5 |
| Product Id | GF-010-5 |
| Product Name | Epidermal Growth Factor (EGF) human (RECOMBINANT) |
| Temperature | +4°C |
| Unit | 100µg |
| Price | 80 |
| Availability | Available |
| Category | Growth Factors, Cytokines, Receptors and their Antibodies |
| Subcategory | Growth Factors/Cytokines/Enzymes |
| Description | Human EGF is produced by genetically engineered yeast, and purified by sequential chromatography and reverse-phase HPLC. This polypeptide is identical to gamma-urogastrone, or equivalent to human EGF1-52. |
| Isotype | N/A |
| Mol Weight | N/A |
| Purity | Over 97% pure by N-terminal amino acid sequencing, amino acid composition, HPLC analysis, and SDS gel electrophoresis. |
| Storage | Store at +4°C. Reconstitution is best in water or buffer near neutral pH. |
| Stability | Several months at +4°C. In solution, at pH 4-7, is stable for 2-3 months at -20°C. |
| Biological Activity | The mitogenic activity of human EGF is measured by the stimulation of 3H-thymidine incorporation into human foreskin fibroblast cells. In addition, EGF activity has been determined by a receptor-binding assay (RBA) using A-431 cells. |
| Formulation | Lyophilized powder |
| References | 1. Cohen, S. (1962) J, Biol. Chem. 237:1555
2. Gregory, H. (1985) J.Cell Science Supp.. 3:11
3.- George-Nascimento, C. et al. (1988) Biochemistry 27:797-802 |
