| Catalog Number | GF-030-3 |
| Product Id | GF-030-3 |
| Product Name | Basic Fibroblast Growth Factor (FGF 2) human (RECOMBINANT) |
| Temperature | +4°C |
| Unit | 10µg |
| Price | 50 |
| Availability | Available |
| Category | Growth Factors, Cytokines, Receptors and their Antibodies |
| Subcategory | Growth Factors/Cytokines/Enzymes |
| Description | Human Basic Fibroblast Growth Factor is a mitogen that stimulates cell growth for such cells as fibroblasts, endothelial cells, myoblasts, glial cells and smooth muscle cells. Basic FGF is composed of 146 amino acid residues (pro 1 to ser 146), and is 55% homologous with acidic FGF, including two conserved cysteine residues. Human basic FGF is produced by genetically engineered yeast, and purified by heparin-Sepharose affinity chromatography. |
| Isotype | N/A |
| Mol Weight | 17.5 KDa |
| Purity | Over 95% pure by N-terminal amino acid sequencing, amino acid composition, HPLC analysis, and SDS gel electrophoresis. |
| Storage | Store at +4°C. Reconstitution is best in water or buffer near neutral pH, since it is unstable in acidic solutions. After reconstitution, storage in aliquots at -80°C is recommended. |
| Stability | Several months, lyophilized or in solution, at pH 6-7 and +4°C. |
| Biological Activity | The mitogenic activity of human basic FGF is identical to natural bovine basic FGF, as measured by stimulation of 3H-thymidine incorporation into human foreskin fibroblast cells. |
| Formulation | Lyophilized powder |
| References | 1. Gospodarowicz, D. (1975) J. Biol. Chem. 250, 2515-2520.
2. Folkman, J. and Klagsbrun, M. (1987) Science 235,442-447
3. Fox, J., et al. (1996) Journal of Biological Chemistry 271: 12578-12584. |
