| Catalog Number | GF-040-4 |
| Product Id | GF-040-4 |
| Product Name | Acidic Fibroblast Growth Factor (FGF 1) human (RECOMBINANT) |
| Temperature | -80°C |
| Unit | 50µg |
| Price | 300 |
| Availability | Available |
| Category | Growth Factors, Cytokines, Receptors and their Antibodies |
| Subcategory | Growth Factors/Cytokines/Enzymes |
| Description | Acidic FGF is a mitogen for cells of mesodermal and neuroectodermal origin, including fibroblasts, endothelial cells, astrocytes, neuroblasts, osteoblasts, and smooth muscle cells. This protein is composed of 140 amino acid residues, and has a 55% homology with basic FGF, including two conserved cysteine residues. Human Acidic FGF is produced by genetically engineered yeast, and purified by heparin-Sepharose affinity chromatography. |
| Isotype | N/A |
| Mol Weight | 15.5 KDa |
| Purity | Over 95% pure by HPLC analysis and SDS gel electrophoresis. |
| Storage | Store at -80°C. It is also possible to keep this protein at low pH (2-5) for a few days. |
| Stability | Unstable without a carrier protein like gelatin or bovine serum albumin. It is recommended to add some of these proteins, prior to aliquoting, for longer storage. |
| Biological Activity | Human acidic FGF is mitogenic for a variety of normal cells in culture including fibroblasts, glial cells, vascular endothelial cells. |
| Formulation | 10 mM Tris-HCl, 1.5 M NaCl, pH 7.0 Concentration: 0.50 mg protein/ml. |
| References | 1. Gimenez-Gallego et al., Biochem. Biophys. Res. Comm.(1986) 138:611
2. Thornton et. al. (1983), Science 222, 623
3. Gospadorowicz, D. (1987) Methods Enzymol. 147:106 |
