| Catalog Number | TM-451-55 |
| Product Id | TM-451-55 |
| Product Name | Anti- human dimethyl - Histone H4 (K20) IgG fraction (monoclonal) |
| Temperature | -20°C |
| Unit | 150µg |
| Price | 425 |
| Availability | Available |
| Category | Proteins and Antibodies for Transcription Research |
| Subcategory | Proteins and Antibodies |
| Description | IgG fraction from mouse monoclonal antibody (clone 7H8/I5) obtained by immunization using a KLH-conjugated synthetic peptide corresponding to aa17 – aa28 of human Histone H4. The IgG fraction was purified using Protein G-Sepharose. |
| Isotype | IgG1 |
| Storage | Store at -20°C. |
| Stability | Stable for at least one year. Avoid repeated freezing and thawing. |
| Biological Activity | This antibody can be used in ELISA assays (1: 8,000 dilution), immunoblot, immunohistochemistry and immunoprecipitation analysis. It recognizes Histone H4 dimethylated on lysine 20. This modification is associated with silent, transcriptionally inactive regions within the euchromatin. This antibody is used to study the mechanisms of regulation of chromatin structure during transcription. |
| Formulation | Solution at 1.0 mg/mL in PBS (pH 7.2). |
| References | Nishioka, K et al. (2002) Mol. Cell. 9, 1201 – 1213. |
