| Catalog Number | GF-070-3 |
| Product Id | GF-070-3 |
| Product Name | Platelet-derived Growth Factor (PDGF-BB) human (RECOMBINANT) |
| Temperature | +4°C |
| Unit | 10µg |
| Price | 100 |
| Availability | Available |
| Category | Growth Factors, Cytokines, Receptors and their Antibodies |
| Subcategory | Growth Factors/Cytokines/Enzymes |
| Description | Human PDGF-BB homodimer is produced by genetically engineered yeast, and purified by sequential chromatography and ionic exchange HPLC. This polypeptide is composed of two identical polypeptide chains attached by disulfide bonds. As it has been described in the natural product, PDGF-BB is partially clipped at Arg32-Thr33. This cleavage is visible upon reduction of the polypeptide with a reducing agent. Note: this clipping does not affect the biological activity. |
| Isotype | N/A |
| Mol Weight | N/A |
| Purity | Over 97% pure by N-terminal amino acid sequencing, amino acid composition, HPLC analysis, and SDS gel electrophoresis. |
| Storage | Store at +4°C. Reconstitution is best in water or buffer near neutral pH. |
| Stability | Several months at +4°C. In solution, at pH 4-7, is stable for 2-3 months at -20°C. |
| Biological Activity | The material is fully active as determined by its mitogenic activity measured by stimulation of 3H-thymidine incorporation into human foreskin fibroblast cells. |
| Formulation | Lyophilized powder |
| References | 1. Ross, R. et al. (1986) Cell 46:155
2. Johnson, A. et al. (1984) EMBO J.3:921
3. Waterfield, M.D. et al. (1983) Nature 303: 35
4. Brake, A.J. et al. (1884) Proc. Natl. Acad. Sci. (USA) 81:4642 |
