| Catalog Number | TM-511-55 |
| Product Id | TM-511-55 |
| Product Name | Anti-human Sirtuin type 3 (Sirt 3). IgG fraction (monoclonal) |
| Temperature | -20°C |
| Unit | 150µg |
| Price | 425 |
| Availability | Available |
| Category | Proteins and Antibodies for Transcription Research |
| Subcategory | Proteins and Antibodies |
| Description | IgG fraction from mouse monoclonal antibody (clone 2D2/H11) obtained by immunization of mice with an ovalbumin-conjugated synthetic peptide corresponding to aa28 - aa77 of human silent information regulator 2 homolog (Sirt3). The IgG fraction was purified using Protein G-Sepharose. |
| Isotype | IgG2b |
| Storage | Store at -20°C. |
| Stability | Stable for at least one year. Avoid repeated freezing and thawing. |
| Biological Activity | This antibody can be used for ELISA assay (1:2,000 dilution). It recognizes Sirt3, a mitochondrial NAD-dependent deacetylase, enzyme involved in the constitutive deacetylation of one or several mitochondrial proteins. The Sirtuin 3 activity could play an important role in the mechanism of the acetylation control of factors directly involved in apoptotic pathways. |
| Formulation | Solution at 0.5 mg/mL in PBS |
| References | Schwer, B. et. al. (2002) J. Cell Biol. 158, 647 - 657.
Smith, J.S. et. al. (2002) Methods Enzymol. 353, 282 - 300.
Frye, R. (2000) Biochem. Biophys. Res. Commun. 273, 793 - 798.
Frye, R. (1999) Biochem. Biophys. Res. Commun. 260, 273 - 279. |
