| Catalog Number | TM-021X-55 |
| Product Id | TM-021X-55 |
| Product Name | Anti-Excision Repair Cross Complement (ERCC2). IgG fraction (monoclonal) |
| Temperature | -20°C |
| Unit | 150µg |
| Price | 425 |
| Availability | Available |
| Category | Proteins and Antibodies for Transcription Research |
| Subcategory | Proteins and Antibodies |
| Description | IgG fraction from mouse monoclonal antibody (clone 2E11/E8) obtained by immunization of mice with a peptide of ERCC2 (80 kDa subunit of TFIIH). The IgG fraction was purified using Protein G-Sepharose. |
| Isotype | IgG2b |
| Storage | Store at -20°C. |
| Stability | Stable for at least one year. Avoid repeated freezing and thawing. |
| Biological Activity | This antibody can be used for ELISA assay (1:8,000 dilution). It recognizes ERCC2 subunit of TFIIH and it has not cross reaction with ERCC3 subunit. ERCC2 is an ATP-dependent 5'-3' DNA helicase, component of the core-TFIIH transcription factor. It is involved in nucleotide excision repair of DNA by opening around the damage, and the initiation of transcription catalyzed by RNA polymerase II. |
| Formulation | Solution at 0.5 mg/mL in PBS |
| References | Schultz, P. et. al. (2000) Cell 105, 599 - 607.
Reardon, J.T. et. al. (1996) Proc. Natl. Acad. Sci. USA 93 6482-
6487.
Drapkin, R. et al (1994) Nature 368, 769 - 772. |
