AUSTRAL Biologicals Catalog

Product Sheet: Human transforming growth factor beta-1 (TGF beta-1) recombinant

Catalog Number	GF-230-2
Product Id	GF-230-2
Product Name	Human transforming growth factor beta-1 (TGF beta-1) recombinant
Temperature	+4°C
Unit	5µg
Price	300
Availability	Available
Category	Growth Factors, Cytokines, Receptors and their Antibodies
Subcategory	Growth Factors/Cytokines/Enzymes
Description	Human Transforming Growth Factor beta1 (TGF- beta1 ) is stimulatory for cells of mesenchymal origin and inhibitory for cells of epithelial or neuroectodermal origin. Mature TGF- beta1 is a disulfide-linked homodimer of two chains composed of 112 amino acid residues each. There is a 70% homology between human TGF- beta1 and human TGF- beta2. Human TGF- beta1 is produced in genetically engineered bacteria, and purified by sequential chromatography.
Isotype	N/A
Mol Weight	25 KDa (dimeric protein)
Purity	Over 98% pure by non-reduced SDS gel electrophoresis.
Storage	Store at +4°C.
Stability	Several months at +4°C.
Biological Activity	The biological activity of human TGF-&# beta1 is determined by measuring the acidification of the culture medium (Rat-1 cells) exposed to the growth factor.
Formulation	25 µl (0.20 mg/ml) in 5 mM HCl (pH 2.5). Some of the liquid in the vial could have been evaporated with changes in the final volume. However, the 5µg of the protein are still inside the vial. We recommend, in order to control the proper concentration, to dilute the entire content to a known volume with 5mM HCl. Do not freeze this material.
References	1. Ichijo, H. et al.(1994) The Journal of Biological Chemistry, Vol 266, No. 33, May, 22459-22464 2. Yan, Z., Winawer, S., and Friedman, E., (1994) The Journal of Biological Chemistry, Vol 269, No. 18, May, 13231-13237.

All products are for research use ONLY in laboratory animals and in vitro testing. NOT for diagnostic or human use.